生物医学工程系学术报告9.17
发布时间: 2010-09-17 12:56:00
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北京大学工学院 生物医学工程系学术报告 |
题目：Probing Nucleosome Function: Systematic Mutagenesis Study of Synthetic Histones in Saccharomyces cerevisiae
JUNBIAO DAI, PH.D.
Johns Hopkins University • School of Medicine
High Throughput Biology Center and Depart of Molecular Biology and Genetics
High Throughput Biology Center and Depart of Molecular Biology and Genetics
时 间： 2010年9月17日下午1:00-2:30
主持人： 席建忠 博士
地 点： 北大校医院A-534
Abstract: The structural integrity of the nucleosome is central to regulation of DNA metabolism and transcription. Using a synthetic approach, a library of 486 systematic histone H3 and H4 substitution and deletion mutants in Saccharomyces cerevisiae was generated that probes the contribution of each residue to nucleosome function and can be episomal or genomically integrated. We probed fitness contributions of each residue to chemical perturbagens of chromosome integrity and transcription, mapping global patterns of chemical sensitivities and requirements for three forms of transcriptional silencing onto the nucleosome surface. Each histone mutant was tagged with unique molecular barcodes, facilitating identification of histone mutant pools through barcode amplification, labeling, and TAG microarray hybridization. We used the barcodes to score complex phenotypes including competitive fitness in a chemostat, DNA repair proficiency, and synthetic genetic interactions, revealing new functions for distinct histone residues and new interdependencies among components of nucleosomes and their modifiers.
Biography
EUCATION
• Post-doctoral Fellow, Department of Molecular Biology and Genetics, Johns Hopkins
University, School of Medicine, Baltimore, MD, September 2006 - current
PROJECT: High-throughput Mutagenesis Analysis of core histones in Saccharomyces
cerevisiae
• Doctor of Philosophy in Molecular, Cellular and Developmental Biology,
Department of Genetics, Development and Cell Biology, Iowa State University
Graduate School, Ames, IA, August 2000 - August 2006
DISSERTATION: Host Control of Integration Specificity of the Ty5 Retrotransposon in
Saccharomyces cerevisiae
• Master of Science in Cell Biology, Department of Biological Science and
Biotechnology, Tsinghua University Graduate School, Beijing, P.R. China, September
1997 - August 2000
THESIS: EPA and DHA Productivity by Marine Microalga Isochrysis galbana
• Bachelor of Science in Biology, Department of Intensive Instruction, Nanjing
University, Nanjing, P.R. China, September 1993 - August 1997
THESIS: The Effects of Chlorophyll Content on the Viscosity of Bio-oil Derived from
Chlorella protothecoides during Heterotrophic Yellowing
ACADEMIC HONORS AND AWARDS
• Recipient, Excellent Graduate in Research, Iowa State University, 2006
• Winner, Sui Tong Chang Fung Award, Iowa State University, 2006
• Recipient, Premium for Academic Excellence (PACE) Award, Iowa State University,
2000
• Recipient, Guanghua Outstanding Graduate Scholarship, Tsinghua University, 1999
• Recipient, Novo Nordisk Outstanding Graduate Biotechnology Award, Tsinghua
University, 1998
• Outstanding Graduate, Nanjing University, 1997
• Outstanding Student Leader, Nanjing University, 1996
• Recipient, Guanghua Outstanding Undergraduate Scholarship, Nanjing University,
1996