生物医学工程系学术报告6.8(报告人：Li YANG ）
发布时间: 2011-06-06 10:09:00
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北京大学工学院 生物医学工程系学术报告 |
Gene targeting and biocore technology in biomedical research
报告人 Li YANG
Beijing Novo Nordisk Pharmaceuticals Science and Technology Co. Ltd, Scientist
报告内容摘要
The effect of the absence of a gene can be very informative about the normal function of the gene. A gene knockout (KO) is a genetic technique in which one of an organism’s genes is made inoperative. Knockout animals can be produced by a technique called gene targeting. This is the replacement of one gene sequence, the sequence resident in the mouse genome, with a related sequence that has been modified in the laboratory to contain a mutation. An overview and the use of this technology will be discussed.
To better understand biomolecular interactions, cutting-edge biosensors are revolutionizing and are established as routine biophysical tools in research laboratories worldwide. Biacore systems are used in areas such as pharmaceutical drug discovery, antibody characterization, proteomics, immunogenicity, biotherapeutic development and manufacture. An introduction for basic principle and some application will be discussed.
主持人：葛子钢 特聘研究员
时间：6月8日（周三）上午8：30
地点：校医院A534会议室
欢迎广大老师和学生参加！
Resume
Li YANG (English name: Linda YANG)
Email: lya（at）novonordisk.com
Nationality: P.R.China
Birthday: 10/29/1975
1) Professional Positions
08/2008-present Beijing Novo Nordisk Pharmaceuticals Science and Technology Co. Ltd, Scientist
04/2007-07/2008 Dana-Farber Cancer Institute, Harvard Medical School, Research Fellow
2) Education
09/2002-03/2007 Children's Hospital Medical Center (CHMC) and the University of Cincinnati, PhD in Molecular and Developmental Biology program
12/1999-08/2002 National University of Singapore, MS in Pediatrics (Allergy and Immunology)
09/1997-12/1999 China Pharmaceutical University, MS candidate in Biological Pharmaceutics
08/1993-06/1997 China Pharmaceutical University, BE in Microbiological Pharmaceutics
3) Honors
1. Oral presentation on 48th Annual Meeting of the American Society of Hematology (ASH), 2006
2. Oral presentation and on 47th Annual Meeting of ASH, 2005
3. Pre-doctoral travel award on 47th Annual Meeting of ASH, 2005
4. 1st prize of 12th Annual Molecular and Developmental Biology Student Symposium, CHMC and University of Cincinnati, 2005
5. Honorable Mention award of Graduate Student Research Forum, University of Cincinnati, 2005
6. 1st prize of Graduate Student Research Forum, University of Cincinnati, 2004
7. Rachford travel award of University of Cincinnati, 2004, 2005, 2006
8. Graduate student travel award, University of Cincinnati, 2005, 2006
9. Best Undergraduate Thesis Award of of China Pharmaceutical University, 1997
10. 1st prize of Undergraduate Scholarship of China Pharmaceutical University, 1994
11. 1st prize of Undergraduate Scholarship of China Pharmaceutical University,1996
4) Peer-reviewed Journal Papers
1. Yang L, Zheng Y. Cdc42: A signal coordinator in hematopoietic stem cell maintenance. Cell Cycle 2007; 6 (12): 1445-50.
2. Yang L, Wang L, Kalfa T, Cancelas JA, Mo J, Williams DA, Zheng Y. Cdc42 is critical for muti-lineage blood cell development from hematopoietic stem cells. Blood 2007 (In revision).
3. Yang L, Wang L, Geiger H, Cancelas JA, Mo J, Zheng Y. Rho GTPase Cdc42 coordinates hematopoietic stem cell quiescence and niche interaction in the bone marrow. PNAS 2007; 104(12): 5091-6. (cited 1 time)
4. Yang L, Wang L, Zheng Y. Gene targeting of Cdc42 and Cdc42GAP affirms the critical involvement of Cdc42 in filopodia induction, directed migration, and proliferation in primary mouse embryonic fibroblasts. MBC 2006; 17(11): 4675-85. (cited 6 times)
5. Wang L, Yang L, Debidda M, Witte D, Zheng Y. Cdc42GAP deficiency promotes genomic instability and premature aging-like phenotype. PNAS 2007; 104(4):1248-53. (cited 2 times)
6. Chen L, Liao GH, Yang L, Campbell K, Nakafuku M, Kuan CY, Zheng Y. Cdc42 deficiency causes Sonic hedgehog-independent holoprosencephaly. PNAS 2006; 103(44): 16520-5. (cited 4 times)
7. Guo F, Debidda M, Yang L, Williams DA, Zheng Y. Genetic deletion of Rac1 GTPase reveals its critical role in actin stress fiber formation and focal adhesion complex assembly. JBC 2006; 281(27): 18652-9. (cited 6 times)
8. Wang L, Yang L, Filippi M, Williams DA, Zheng Y. Genetic deletion of Cdc42GAP reveals a role of Cdc42 in erythropoiesis and in hematopoietic stem/progenitor cell survival, adhesion and engraftment. Blood 2006; 107(1): 98-105. (cited 12 times)
9. Nusser N, Gosmanova E, Makarova N, Fujiwara Y, Yang L, Guo F, Luo Y, Zheng Y, Tigyi G. Serine phosphorylation differentially affects RhoA binding to effectors: implications to NGF-induced neurite outgrowth. Cell Signal 2006; 18(5): 704-14. (cited 5 times)
10. Wang L, Yang L, Burns KA, Kuan CY, Zheng Y. Cdc42GAP regulates c-Jun N-terminal kinase (JNK)-mediated apoptosis and cell number during mammalian perinatal growth. PNAS 2005; 102 (38): 13484-9. (cited 13 times)
11. Zhang B, Yang L, Zheng Y. Novel intermediate of Rac GTPase activation by guanine nucleotide exchange factor. BBRC 2005; 331(2): 413-21. (cited 2 times)
12. Lim MA, Yang L, Zheng Y, Wu H, Dong LQ, Liu F. Roles of PDK-1 and PKN in regulating cell migration and cortical actin formation of PTEN-knockout cells. Oncogene 2004; 23(58): 9348-58. (cited 7 times)
13. Wang L, Yang L, Luo Y, Zheng Y. A novel strategy for specifically down-regulating individual Rho GTPase activity in tumor cells. JBC 2003; 278 (45): 44617-25. (cited 25 times)
14. Yang L, Cheong N, Wang DY, Lee BW, Kuo IC, Huang CH, Chua KY. Generation of monoclonal antibodies against Blo t3 using DNA immunization with in vivo electroporation. Clin Exp Allergy 2003; 33(5): 663-8. (cited 11 times)
15. Cheong N, Yang L, Lee BW, Chua KY. Cloning of a group 3 allergen from Blomia tropicalis mites. Allergy 2003; 58(4):352-6. (cited 11 times)
16. Xin M, Yang L, Jie L, Min D, Liu J. An X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis: cloning, expression in Escherichia coli, and application for removal of N-terminal Pro-Pro from recombinant proteins. Protein Expr Purif 2002; 24(3): 530-8. (cited 2 time)
5) Conference Papers
1. Yang L, Wang L, Zheng Y. Cdc42 is critical for muti-lineage blood cell development from hematopoietic stem cells. 5th Annual Midwest Blood Club Meeting, 2007.
2. Yang L, Wang L, Geiger H, Cancelas JA, Williams DA, Zheng Y. Cdc42 is critical for multi-lineage blood cell development from hematopoietic stem cells. Blood 2006; 108 (11): 191A-191A 633 Part 1 NOV 16 (Selected for Oral presentation at 48th ASH Annual meeting, 2006.)
3. Yang L, Wang L, Cancelas JA, Williams DA, Zheng Y. A critical role of the Rho Family GTPase Cdc42 in hematopoietic stem cell mobilization, homing, engraftment and differentiation. Blood 2005; 106 (11): 82A-82A 269 Part 1 NOV 16. (Selected for Oral presentation at 47th ASH Annual meeting, 2005)
4. Wang L, Yang L, Burns KA, Kuan CY, Zheng Y.Cdc42GAP regulates mammalian perinatal growth and aging. Annual symposium of Ryan fellows, 2005. (Selected for Oral presentation)
5. Wang L, Yang L, Burns KA, Kuan CY, Zheng Y.Cdc42GAP regulates mammalian perinatal growth and aging. Annual symposium of Ryan fellows, 2005. (Oral)
6. Yang L, Wang L, Zheng Y. Cdc42 loss function mouse revealed a critical role in hematopoietic stem/progenitor cell engraftment and differentiation. 3rd Annual Midwest Blood Club Meeting, 2005
7. Wang L, Yang L, Filippi M, Williams DA, Zheng Y. Cdc42 regulates multiple hematopoietic stem/progenitor cell functions and erythropoiesis. Blood 2004; 104 (11): 13A-13A 32 Part 1 NOV 16. (Selected for Oral presentation at 46th ASH Annual meeting, 2004)
8. Wang L, Yang L, Zheng Y. Cdc42 regulates mouse embryonic and postnatal growth by mediating an apoptosis signal that controls cell number. FASEB Summer Research Conferences, 2004. (Selected for Oral presentation)
6) Professional Membership
Active member of The American Association for Cancer Research